CELL CHIPS


CELL CHIPS FOR 2D CELL-BASED ASSAYS

  • ANISOTROPIC ON-CHIP PROTEIN PATTERNING
In cooperation with Prof. Seta Küpcü (Department for Nanobiotechnology, University of Natural Resources and Life Sciences, Vienna) we have developed a tunable, facile and reliable cell patterning method using  self-assembled bacterial crystalline protein monolayers (S-layers) that, depending on its orientation or host origin, can exhibit either cell adhesive (cytophilic) or cell repulsive (cytophobic) surface properties. Laminar flow patterning was applied to generate anisotropic surfaces for on-chip cellular micropatterning.






  • CELL-CELL INTERACTION
We have developed, characterized and evaluated a complementary cell analysis method to assess the dynamic interactions of tumor cells with resident tissue and immune cells using optical light scattering and impedance spectroscopy to shed light on tumor cell behavior.







  • ON-CHIP NANOTOXICOLOGY
The developed lab-on-a-chip is capable of non-invasively monitoring changes in the production of extracellular matrix components (ECM) such as the deposition of collagen fibers by normal human dermal fibroblasts (NHDF). We showed the successful culturing of NHDF in a microfluidic channel system and the simultaneous non-invasive monitoring of the impact of NP onto ECM production.






  • FLOW INDUCED NANOMATERIAL UPTAKE:
Similarliy to our nanotoxicology on-chip lung model, fluid dynamics is also known to influence intracellular uptake of nanomaterials. Here, we have developed a endothelial cell model for on-chip nanoparticle uptake studies.

  


CELL CHIPS FOR 3D CELL-BARRIER MODELSe
  • MEMBRANE-FREE HYDROGEL CELL CHIPS
Section under construction...



  • MEMBRANE-BOUND CELL-BARRIER CHIPS
Section under construction...




  • MUTLI-MEMBRANE TISSUE CHIPS
Section under construction...


OTHER CELL CHIP SYSTEMS

  • FUNGAL BIOFILM ANALYSIS:
We reported the development of a disposable microfluidic biochip capable of continuously monitoring cell population dynamics under physiological shear force conditions. We demonstrate the simultaneous application of contact-less bioimpedance spectroscopy and amperometric measurements to monitor fungal biofilm growth rates and metabolic activities. 




  • ADVANCED VIRAL INFECTIVITY ASSAY:
Cell-based infectivity assays are routinely performed to determine the presence of viral infections or to demonstrate viral clearance. Our extended infectivity assay is based on simultaneous cellular virus amplification and cellular virus recognition by combining two microfluidic cell cultures on a single chip platform.