Vascularization on chip

Establishment of vascular networks in biochips using co-cultures of adipose derived stem cells and endothelial cells in a 3D fibrin matrix
T
he development of novel vascularization strategies for tissue constructs is still one of the major challenges in tissue engineering and regeneration. In the present study we investigate the establishment of vascular networks in the absence and presence of fluid flow conditions using a microfluidic co-culture system. In the presented configuration adipose derived stem cells (ASCs) and human umbilical vein cells (HUVEC) are embedded within a 3D fibrin matrix to study accumulation and depletion of ASCs secreted paracrine pro-angiogenic factors on vascular tube formation